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Western blot(SDS PAGE) analysis of extracts from A549 cell lysate.Using ADORA1 Rabbit mAb [0a03]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :A1AR; A1R; AA1R; Adenosine receptor A1; ADORA 1; ADORA1; RDC 7; Ri;
UniProtID :P30542
MASS(da) :36,512
MW(kDa) :37kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ADORA1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cell lysate.
Function : Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.