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Western blot(SDS PAGE) analysis of extracts from human fetal heart lysate.Using Superoxide Dismutase 3 Rabbit mAb [wEVk]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EC SOD; Extracellular superoxide dismutase [Cu Zn]; Extracellular superoxide dismutase precursor; SOD3; Superoxide dismutase 3 extracellular;
UniProtID :P08294
MASS(da) :25,851
MW(kDa) :26kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Superoxide Dismutase 3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal heart lysate.
Function : Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Tissue specificity :Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Subcellular locationi :Secreted, extracellular space. Golgi apparatus, trans-Golgi network.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.