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Western blot(SDS PAGE) analysis of extracts from (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.Using Dynamin 1 Rabbit mAb [5YUD]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :B dynamin; D100; DNM 1; DNM1; Dynamin; Dynamin1;
UniProtID :Q05193
MASS(da) :97,408
MW(kDa) :97kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Dynamin 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.
Function : Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.