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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Ferritin Heavy Chain Rabbit mAb [Ei29]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Apoferritin; F HC; Ferritin H subunit; FHC; FRIH; FTH; FTH1; FTHL6; N-terminally processed; PIG15; PLIF;
UniProtID :P02794
MASS(da) :21,226
MW(kDa) :21kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Ferritin Heavy Chain
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity)..
Tissue specificity :Expressed in the liver..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.