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Muscarinic Acetylcholine Receptor M3 Rabbit mAb [Af1h]Cat NO.: A95326

Western blot(SDS PAGE) analysis of extracts from human fetal brain lysate.Using Muscarinic Acetylcholine Receptor M3 Rabbit mAb [Af1h]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :AChR; CHRM3; EGBRS; HM3; M3 muscarinic receptor;

UniProtID :P20309

MASS(da) :66,128

MW(kDa) :66kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human fetal brain lysate.

Function : The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover..

Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane,Multi-pass membrane protein. Basolateral cell membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.