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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using IKB epsilon Rabbit mAb [wNAq]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :I-kappa-B-epsilon; IkappaBepsilon; IkB-E; IkB-epsilon; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFKBIE;
UniProtID :O00221
MASS(da) :52,864
MW(kDa) :45kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human IKB epsilon
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes..
Tissue specificity :Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.