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Western blot(SDS PAGE) analysis of extracts from HT-1080 cell lysate.Using TAX1BP3 Rabbit mAb [2J4j]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Glutaminase interacting protein 3; Tax interaction protein 1; Tax1 binding protein 3; Tax1bp3; TIP1;
UniProtID :O14907
MASS(da) :13,735
MW(kDa) :14kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TAX1BP3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-1080 cell lysate.
Function : May regulate a number of protein-protein interactions by competing for PDZ domain binding sites. Binds CTNNB1 and may thereby act as an inhibitor of the Wnt signaling pathway. Competes with LIN7A for KCNJ4 binding, and thereby promotes KCNJ4 internalization. May play a role in the Rho signaling pathway. May play a role in activation of CDC42 by the viral protein HPV16 E6..
Tissue specificity :Ubiquitous. Detected in brain, heart, kidney, lung, small intestine and skeletal muscle. Detected in various cell lines including HeLa. Weakly expressed in peripheral blood leukocytes..
Subcellular locationi :Cytoplasm. Nucleus. Cell membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.