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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using Factor X Rabbit mAb [kEca]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Activated factor Xa heavy chain; Coagulation factor X; F10; factor Xa;FXA; Prothrombinase; Stuart factor;
UniProtID :P00742
MASS(da) :54,732
MW(kDa) :74kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Factor X
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificity :Plasma,synthesized in the liver..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.