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Western blot(SDS PAGE) analysis of extracts from (1) K562 cell lysate; (2) Raw264.7 cell lysate.Using SA2 Rabbit mAb [nj92]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SA 2; SA-2; SA2; SCC3 homolog 2; SCC3B; stag2; Stromal antigen 2;
UniProtID :Q8N3U4
MASS(da) :141,326
MW(kDa) :141kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SA2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) K562 cell lysate; (2) Raw264.7 cell lysate.
Function : Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis..
Subcellular locationi :Nucleus. Chromosome. Chromosome, centromere.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.