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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using Galectin 1 Rabbit mAb [o9HE]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GAL1; Galaptin; Galbp; Galectin; Galectin1; GBP; HBL; HLBP14; HPL; L14; Lect14; LGALS1;
UniProtID :P09382
MASS(da) :14,716
MW(kDa) :15kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Galectin 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis..
Tissue specificity :Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells..
Subcellular locationi :Secreted, extracellular space, extracellular matrix. Cytoplasm. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.