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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb [4V0N]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :pdhB; DHBD; PDHE1 B; PDHE1-B; PHE1B;
UniProtID :P11177
MASS(da) :39,233
MW(kDa) :35kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle..
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.