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LMAN1 Rabbit mAb [I4JV]Cat NO.: A49581

Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) Jurkat cell lysate.Using LMAN1 Rabbit mAb [I4JV]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ERGIC5; F5F8D; FMFD1; Gp58; Lman1; MCFD1; MR60;

UniProtID :P49257

MASS(da) :57,549

MW(kDa) :58kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human LMAN1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) HeLa cell lysate; (2) Jurkat cell lysate.

Function : Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins..

Tissue specificity :Ubiquitous..

Subcellular locationi :Endoplasmic reticulum-Golgi intermediate compartment membrane,Single-pass type I membrane protein. Golgi apparatus membrane,Single-pass membrane protein. Endoplasmic reticulum membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.