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Western blot(SDS PAGE) analysis of extracts from Ramos cell lysate.Using eIF2A Rabbit mAb [Mw01]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :65 kDa eukaryotic translation initiation factor 2; Eukaryotic translation initiation factor 2A; ACDA02; EIF 2A; CDA02; MSTP004; MSTP089; EIF2; eif2a; eIF-2A
UniProtID :Q9BY44
MASS(da) :64,990
MW(kDa) :65kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human eIF2A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cell lysate.
Function : Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner..
Tissue specificity :Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.