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Western blot(SDS PAGE) analysis of extracts from Neuro-2a cell lysate.Using VGLUT1 Rabbit mAb [s3lc]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BNPI; Slc17a7; VGluT1;
UniProtID :Q9P2U7
MASS(da) :61,613
MW(kDa) :61kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human VGluT1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Neuro-2a cell lysate.
Function : Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate..
Tissue specificity :Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe..
Subcellular locationi :Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane,Multi-pass membrane protein. Cell junction, synapse, synaptosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.