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Western blot(SDS PAGE) analysis of extracts from Molt4 cell lysate.Using DNA Ligase I Rabbit mAb [MO9l]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DNA ligase 1; DNA ligase I; lig1;
UniProtID :P18858
MASS(da) :101,736
MW(kDa) :140kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human DNA Ligase I
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Molt4 cell lysate.
Function : DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.