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Western blot(SDS PAGE) analysis of extracts from Human small intestine lysate.Using Mast Cell Chymase Rabbit mAb [726o]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Chymase 1; Chymase; CMA1; CYH; CYM; Mcp-5; MCP3P; Mcpt5; MCT1; MMCP-5;
UniProtID :P23946
MASS(da) :27,325
MW(kDa) :27kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Mast Cell Chymase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human small intestine lysate.
Function : Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Tissue specificity :Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions..
Subcellular locationi :Secreted. Cytoplasmic granule.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.