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Western blot(SDS PAGE) analysis of extracts from Red blood cell lysate.Using Band 3 / CD233 Rabbit mAb [JWUk]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AE1; BND3; CD233; DI; EMPB3; EPB3; FR; RTA1A; SLC4A1; SW; WD; WD1; WR;
UniProtID :P02730
MASS(da) :101,792
MW(kDa) :102kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Band 3 / CD233
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Red blood cell lysate.
Function : Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane,required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine..
Tissue specificity :Detected in erythrocytes (at protein level)..,[Isoform 2]: Expressed in kidney (at protein level)..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Basolateral cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.