Furin Rabbit mAb [b9W2]Cat NO.: A60259

Protein names ：FUR; FURIN; PACE; PCSK3; SPC1;

UniProtID ：P09958

MASS(da) ：86,678

MW(kDa) ：87kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human Furin

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HepG2 cell lysate.

Function ： Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771, PubMed:2251280, PubMed:24666235, PubMed:25974265, PubMed:7592877, PubMed:7690548, PubMed:9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed:7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed:20489134, PubMed:21763278). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (By similarity).., (Microbial infection) Cleaves and activates diphtheria toxin DT.., (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA).., (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin.., (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP.., (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells.., (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F..

Tissue specificity ：Seems to be expressed ubiquitously..

Subcellular locationi ：Golgi apparatus, trans-Golgi network membrane,Single-pass type I membrane protein. Cell membrane,Single-pass type I membrane protein. Secreted. Endosome membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.