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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using RCAS1 Rabbit mAb [4BUY] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EBAG9,RCAS1,RCAS1_HUMAN,Receptor-binding cancer antigen expressed on SiSo cells
UniProtID :O00559
MASS(da) :24,377
MW(kDa) :32 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human RCAS1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases..
Tissue specificity :Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
Subcellular locationi :Golgi apparatus membrane,Single-pass type III membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.