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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using Transferrin Receptor 2 Rabbit mAb [5l1K]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HFE3; TfR2; TFRC2;
UniProtID :Q9UP52
MASS(da) :88,755
MW(kDa) :100kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Transferrin Receptor 2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
Tissue specificity :Predominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly.
Subcellular locationi :Cell membrane,Single-pass type II membrane protein.,[Isoform Beta]: Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.