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Western blot(SDS PAGE) analysis of extracts from MCF7 cell lysate.Using DDAH2 Rabbit mAb [Y8Nw]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DDAH; DDAH II; DDAH2; DDAHII; Dimethylargininase 2; G6a;
UniProtID :O95865
MASS(da) :29,644
MW(kDa) :30kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human DDAH2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 cell lysate.
Function : Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation..
Tissue specificity :Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain..
Subcellular locationi :Cytoplasm. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.