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Western blot(SDS PAGE) analysis of extracts from (1) 293 cell lysate; (2) Mouse spleen lysate; (3) C6 cell lysate.Using HES5 Rabbit mAb [c84A]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HES 5; Hes5;
UniProtID :Q5TA89
MASS(da) :18,226
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HES5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) 293 cell lysate; (2) Mouse spleen lysate; (3) C6 cell lysate.
Function : Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator (By similarity)..
Tissue specificity :Expressed in fetal heart and brain tumors..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.