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SPI1 Rabbit mAb [8Hm2]Cat NO.: A70393

Western blot(SDS PAGE) analysis of extracts from Daudi cell lysate.Using SPI1 Rabbit mAb [8Hm2]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :oncogene spi1; PU.1; SFPI1; SPI 1; SPI A; Spi1;

UniProtID :P17947

MASS(da) :31,083

MW(kDa) :42kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PU.1/Spi1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Daudi cell lysate.

Function : Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.