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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Nucleostemin Rabbit mAb [fN92]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :E2IG3; GNL3; NNP47; NS; Nucleostemin;
UniProtID :Q9BVP2
MASS(da) :61,993
MW(kDa) :65kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Nucleostemin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity)..
Tissue specificity :Increased levels in lung tissue in cancer patients..
Subcellular locationi :Nucleus. Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.