PIST Rabbit mAb [NevL]Cat NO.: A18340

Protein names ：GOPC; CAL; CFTR-associated ligand; GOPC1; FIG; PIST;

UniProtID ：Q9HD26

MASS(da) ：50,520

MW(kDa) ：51kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human PIST

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：A375 cell lysate.

Function ： Plays a role in intracellular protein trafficking and degradation (PubMed:11707463, PubMed:14570915, PubMed:15358775). May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels (By similarity). May also regulate the intracellular trafficking of the ADR1B receptor (PubMed:15358775). May play a role in autophagy (By similarity). Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR (PubMed:23818989). Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes (PubMed:11707463, PubMed:14570915)..

Tissue specificity ：Ubiquitously expressed..

Subcellular locationi ：Cytoplasm. Golgi apparatus membrane,Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane,Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendrite.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.