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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using Carbonic Anhydrase 1 Rabbit mAb [0iaX]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CA-I; Carbonic anhydrase B; CAB; Car1;
UniProtID :P00915
MASS(da) :28,870
MW(kDa) :29kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat Zebrafish
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Carbonic Anhydrase 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Catalyzes the reversible hydration of carbon dioxide (PubMed:10550681, PubMed:18618712). Can hydrate cyanamide to urea (PubMed:10550681)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.