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p60 CAF1 Rabbit mAb [F93v]Cat NO.: A12354

Western blot(SDS PAGE) analysis of extracts from Caco2 cell lysate.Using p60 CAF1 Rabbit mAb [F93v]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CAF 1A; CAF IP60; CAF-1; CAF1; CAF1A; CAF1P60; CHAF1B; MPHOSPH7; MPP7;

UniProtID :Q13112

MASS(da) :61,493

MW(kDa) :70kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human p60 CAF1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Caco2 cell lysate.

Function : Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA,histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer..

Subcellular locationi :Nucleus. Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.