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Western blot(SDS PAGE) analysis of extracts from Caco2 cell lysate.Using p60 CAF1 Rabbit mAb [F93v]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CAF 1A; CAF IP60; CAF-1; CAF1; CAF1A; CAF1P60; CHAF1B; MPHOSPH7; MPP7;
UniProtID :Q13112
MASS(da) :61,493
MW(kDa) :70kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human p60 CAF1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Caco2 cell lysate.
Function : Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA,histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.