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Western blot(SDS PAGE) analysis of extracts from (1) MCF7 cell lysate; (2) RAW264.7 cell lysate.Using MC4 Receptor Rabbit mAb [fhY1]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MC4R; Melanocortin 4 receptor;
UniProtID :P32245
MASS(da) :36,943
MW(kDa) :37kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human MC4 Receptor
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) MCF7 cell lysate; (2) RAW264.7 cell lysate.
Function : Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP)..
Tissue specificity :Brain, placental, and gut tissues.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.