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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Desmoglein 2 Rabbit mAb [ACYg]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :desmoglein-2; DSG2; HDGC; ARVD10; CDHF5; CMD1BB;
UniProtID :Q14126
MASS(da) :122,294
MW(kDa) :160kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Desmoglein 2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificity :All of the tissues tested and carcinomas.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Cell junction, desmosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.