E2F4 Rabbit mAb [108h]Cat NO.: A46495

Protein names ：E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; E2F4;

UniProtID ：Q16254

MASS(da) ：43,960

MW(kDa) ：60kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human E2F4

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Raji cell lysate.

Function ： Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis..

Tissue specificity ：Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Subcellular locationi ：Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.