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Western blot(SDS PAGE) analysis of extracts from Human fetal heart lysate.Using Cardiac Troponin C Rabbit mAb [Hi12]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :tnnc1a; TNC; Tnnc1; TNNI3; Troponin C;
UniProtID :P63316
MASS(da) :18,403
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cardiac Troponin C
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal heart lysate.
Function : Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.