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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using PEDF Rabbit mAb [lJSr]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EPC1; OI12; OI6; PEDF; PIG35; Serpinf1;
UniProtID :P36955
MASS(da) :46,312
MW(kDa) :46kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PEDF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Neurotrophic protein,induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity..
Tissue specificity :Retinal pigment epithelial cells and blood plasma..
Subcellular locationi :Secreted. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.