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Western blot(SDS PAGE) analysis of extracts from 0.Using Uroplakin3a Rabbit mAb [Riee]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :UP3a; UPIII; UPIIIA; UPK3; UPK3A; Uroplakin3A; UroplakinIII;
UniProtID :O75631
MASS(da) :30,670
MW(kDa) :kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Uroplakin III
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
Function : Component of the asymmetric unit membrane (AUM),a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity)..
Tissue specificity :Expressed in ureter..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.