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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using Cytokeratin 6 Rabbit mAb [PNZE]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CK 6A; CK 6B; CK 6C; CK 6D; CK 6E; CK-6C; CK-6E; K6a keratin; K6b keratin; K6C; K6c keratin; K6d keratin; K6e keratin; Keratin; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E;
UniProtID :P02538/P04259/P48668
MASS(da) :60,045
MW(kDa) :60kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CK6
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Function : Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair..
Tissue specificity :Expressed in the corneal epithelium (at protein level)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.