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CD53 Rabbit mAb [d17y]Cat NO.: A14139

Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using CD53 Rabbit mAb [d17y]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CD53; CD53 antigen; MOX44; OX44; RATOX44; Tetraspanin25; Transmembrane glycoprotein; TSPAN25; PRO1340;

UniProtID :P19397

MASS(da) :24,341

MW(kDa) :24kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CD53

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cell lysate.

Function : Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity)..

Tissue specificity :B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.

Subcellular locationi :Cell membrane. Cell junction. Membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.