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Western blot(SDS PAGE) analysis of extracts from TOV-112D cells.Using ROBO2 Rabbit mAb [5UG5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ROBO2,KIAA1568,ROBO2_HUMAN,Roundabout homolog 2
UniProtID :Q9HCK4
MASS(da) :151,200
MW(kDa) :180, 220 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ROBO2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :TOV-112D cells
Function : Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.