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Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using TDT Rabbit mAb [4y8c]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DNA nucleotidylexotransferase; DNTT; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase;
UniProtID :P04053
MASS(da) :58,536
MW(kDa) :59kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TDT
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cell lysate.
Function : Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.