Kininogen 1 Rabbit mAb [5Kle]Cat NO.: A47698

Protein names ：BDK; Bradykinin; HMWK; Kallidin I; Kallidin II; KNG; KNG1; WILLIAMS FACTOR;

UniProtID ：P01042

MASS(da) ：71,957

MW(kDa) ：56,130kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human Kininogen 1

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Human platelet lysate.

Function ： (1) Kininogens are inhibitors of thiol proteases,(2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII,(3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes,(4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action),(5) LMW-kininogen inhibits the aggregation of thrombocytes,(6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

Tissue specificity ：Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors..

Subcellular locationi ：Secreted, extracellular space.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.