Brachyury Rabbit mAb [Toxu]Cat NO.: A56386

Protein names ：Brachyury homolog; Bry; Protein T; SAVA; TFT; T-box transcription factor T; TBXT; Brachyury protein;

UniProtID ：O15178

MASS(da) ：47,443

MW(kDa) ：47,41kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Mouse, Rat, Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human Brachyury

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：MUG-Chor1 (human sacral bone chordoma) cell lysate.

Function ： Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site..

Tissue specificity ：Detected in testis, but not in other, normal tissues. Detected in lung tumors (at protein level)..

Subcellular locationi ：Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.