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Western blot(SDS PAGE) analysis of extracts from Rat heart lysate.Using NDUFS4 Rabbit mAb [1eBG]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AQDQ; CI 18; CI AQDQ; Complex I 18 kDa; NDUFS4;
UniProtID :O43181
MASS(da) :20,108
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Mouse, Rat, Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NDUFS4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat heart lysate.
Function : Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.