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Western blot(SDS PAGE) analysis of extracts from human skeletal muscle lysate.Using Alpha actinin 2 Rabbit mAb [zbgG]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Actin binding protein; Actinin alpha 2; ACTN2; Alpha actinin 2; CMD1AA;
UniProtID :P35609
MASS(da) :103,854
MW(kDa) :103kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Mouse, Rat, Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Alpha actinin 2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human skeletal muscle lysate.
Function : F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificity :Expressed in both skeletal and cardiac muscle.
Subcellular locationi :Cytoplasm, myofibril, sarcomere, Z line.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.