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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using TRPC1 Rabbit mAb [WD7X]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HTRP1; Mtrp1; TRP1; TRPC1; Trrp1;
UniProtID :P48995
MASS(da) :91,212
MW(kDa) :120kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TRPC1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion..
Tissue specificity :Seems to be ubiquitous.
Subcellular locationi :Membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.