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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using SEC61A Rabbit mAb [29x9]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :fb62c11; HSEC61; SEC 61; SEC 61A; Sec61 alpha 1; SEC61; SEC61A1;
UniProtID :Q9H9S3
MASS(da) :52,248
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SEC61A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Function : Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins (By similarity)..
Subcellular locationi :Endoplasmic reticulum membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.