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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using RAP1GAP Rabbit mAb [70Ub]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAP1GA1; Rap1GAP; Rap1GAP1; RAP1GAPII; RAPGAP;
UniProtID :P47736
MASS(da) :73,361
MW(kDa) :95kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human RAP1GAP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state..
Tissue specificity :Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues..
Subcellular locationi :Golgi apparatus membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.