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Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using TC10 Rabbit mAb [uc8e]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ARHQ; RASL7A; Rho related GTP binding protein RhoQ; Rhoq; TC10A;
UniProtID :P17081
MASS(da) :22,659
MW(kDa) :25 kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TC10
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cell lysate.
Function : Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia..
Subcellular locationi :Cytoplasm. Cell membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.