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Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using Calpain small subunit 1 Rabbit mAb [rdsD]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Calpain4; CalpainS1; CANP; CANPS; CAPN4; CAPNS; Capns1; CDPS; CSS1;
UniProtID :P04632
MASS(da) :28,316
MW(kDa) :28 kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Calpain small subunit 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cell lysate.
Function : Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity)..
Subcellular locationi :Cytoplasm. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.