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PKA R2 Rabbit mAb [2JPa]Cat NO.: A50095

Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using PKA R2 Rabbit mAb [2JPa]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :KAP2; PKR2; PRKAR2; PRKAR2A;

UniProtID :P13861

MASS(da) :45,518

MW(kDa) :52kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human Cow

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PKA R2

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K562 cell lysate.

Function : Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Tissue specificity :Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Subcellular locationi :Cytoplasm. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.