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Western blot(SDS PAGE) analysis of extracts from HT-29 cell lysate.Using GPA33 Rabbit mAb [4zG4]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Cell surface A33 antigen; Gpa33;
UniProtID :Q99795
MASS(da) :35,632
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GPA33
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cell lysate.
Function : May play a role in cell-cell recognition and signaling.
Tissue specificity :Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.