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Western blot(SDS PAGE) analysis of extracts from (1) SH-SY5Y cell lysate; (2) C6 cell lysate.Using NRG3 Rabbit mAb [4MTw]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HRG3; Neuregulin 3; Nrg3; Pro neuregulin 3;
UniProtID :P56975
MASS(da) :77,901
MW(kDa) :56kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NRG3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) SH-SY5Y cell lysate; (2) C6 cell lysate.
Function : Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes..
Tissue specificity :Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
Subcellular locationi :[Pro-neuregulin-3, membrane-bound isoform]: Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.