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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using ABAT Rabbit mAb [LrOV]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ABAT; GABA transaminase; GABA transferase; GABAT; LAIBAT;
UniProtID :P80404
MASS(da) :56,439
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ABAT
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively (PubMed:10407778, PubMed:15528998). Can also convert delta-aminovalerate and beta-alanine (By similarity)..
Tissue specificity :Liver > pancreas > brain > kidney > heart > placenta..
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.